Mechanism of Receptor-Interacting Protein 3 on Tumor Necrosis Factor Alpha in Gallbladder Cancer Cells

To investigate the mechanism of receptor-interacting protein 3 on proliferation, invasion and reactive oxygen species levels gallbladder cancer cells induced by tumor necrosis factor alpha. Gallbladder SGC-996 were divided into a control group, plasmid cloning deoxyribonucleic acid-negative acid-receptor-interacting 3+tumor alpha group 3+si-receptor-interacting group. Reverse transcription polymerase chain reaction for messenger ribonucleic acid expression in cells; detection cell counting kit-8, Transwell flow cytometry, respectively; 3, matrix metallopeptidase-9, metallopeptidase-2 proteins (Western blotting method). The was lower than epithelial (p<0.05) lowest (p<0.05). acid, survival rate, ability, level, receptorinteracting metallopeptidase-9 similar groups (p>0.05), while protein, content elevated. And survival, decreased acid-receptorinteracting Compared with increased 3+sireceptor interacting Overexpression inhibited proliferation promoted its related to expression.